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Life Science Applications UV Visible Spectrophotometer Evolution 260 Bio
The built-in application module simplifies daily nucleic acid and protein analysis experiments, nucleic acid concentration and purity analysis • DNA,
Product details
We offer a variety of intelligent accessories for you to choose from for life science applications UV visible spectrophotometers to meet your special needs
The attachments of 7-pool and 8-pool converters ensureLarge sample quantity analysis
Temperature control accessories not only enable your samplesProtected from environmental fluctuations, it can also enhance your experimental abilities
Intelligent liquid suction device eliminates time-consuming sample transferstep
Simple application programObtain research level experimental resultsSimplify routine analysis using Bio applications
Simplify daily nucleic acid and protein analysis experiments using built-in application modules. The Bio application module is easy to operate and provides you with quick guidance. Through the INSIGHT Bio application, you can:
Collect the full spectrum after each measurement, analyze the data, and report the results
Automatically use correction factors and custom equations to avoid calculation errors
Automatically save data, with the option to choose from multiple data export formats (XML, CSV, or TSV), orUsers can send emails to designated accounts for offline data processing or storage
By inputting specific nucleic acid sequences and features (such as phosphorylation level), the built-in OligaCalculate molecular weight, extinction coefficient, concentration factor, and melting point in the calculator
Quick measurement and preview
The real-time display function of INSIGHT software enables real-time measurement or peak height of samplesQuick recognition has become simpler. Display your test results in absorbance or transmission mode and print them out as a record.
Direct absorbance testMeet your daily testing needs
All standard testing procedures used to evaluate test samples are pre made, making your life easier. The sample concentration can be determined by simple ratio or wavelength scanning. The direct absorbance test includes:
• Nucleic acid concentration and purity analysis
• DNA, RNA, ssDNA, and Oligos (A260/A280, A260/A230) • Protein concentration and purity analysis (A260/A280)
Fluorescence labeling efficiency of proteins and nucleic acids
OD600 for cell culture
Colorimetric determination of non pure protein samples
Due to buffer solutions or other UV absorbing components, some protein samples cannot be directly read for absorbance (such as cell lysis and non pure protein extraction experiments). In this case, colorimetric analysis can be used for protein quantification. We provide you with several of the most common colorimetric prefabrication applications currently available, eliminating the need for tedious method configuration steps.
By using the convenient Thermo Scientific ™ Pierce ™ BCA ™ Protein, Pierce Modified Lowry or Pierce 660 nm protein detection reagents, pre prepared in Evolution 260 BioThe Cheng method provides a convenient automated protein concentration analysis. Combining instruments and reagents can help ensure the accuracy and reliability of each analysis.
|
reagent |
describe |
wavelength |
compatibility |
|
Pierce 660 |
•Use specialized detection dyes •Faster，And compared to traditional experimental methodsCompared to having a larger linear response •Reaching a stable endpoint |
•exist660 nmmeasure •exist750 nmcalibration |
•Can be used with reducing agents、Chelating agentCompatible with most detergents •giveSDSWash with most ionsCompatibility reagents are required when using polyester agents together |
|
Pierce BCA |
•Using Quinoline Formic Acid（BCA） •Although this experiment is conducted within a large concentration rangeThe circumference is linear，But it is best to represent the calibration curve as second-order •The differences between different protein samples are relatively small |
•exist562 nmmeasure•exist750 nmcalibration |
•Compatible with most detergents •Not recommended with reducing agents、sulfurAlcohol and chelating agent used together |
|
Pierce Modified LowrY reagent |
• Using copper sulfate in alkaline solution •With peptides（Three amino acids or more）mixclose •Reagents need to be added regularly，Longer trialTesting time |
•exist650nmmeasure •exist405 nmcalibration |
•Can be withSDScompatible •Not recommended with reducing agents、selfUsed together with thiols and chelating agents |
|
BradfordSimakao |
•Using Coomassie blue dye •Using ready-made formulas for simple and fasttest •Color response is affectedpHAnd temperature influence •Protein must> 3000 Da |
•exist 595nmmeasure •exist 750 nmcalibration |
•Can be used with most reducing agents and sulfurAlcohol compatibility •Not recommended for use with surfactantsUse together |
|
proteinBiuret |
•Using copper sulfate under alkaline conditions •givePierce Modified Lowrytry Inspection each other be similar，But this experiment requires more protein |
•exist 545 nmplace measure measure •exist 750 nmcalibration |
•Can be used with most reducing agents andThiol compatible •It is not recommended to use it together with ammonium saltsuse |
Dynamics research based on time and temperature:
A dynamic integrated solution consisting of comprehensive software and accessories can quickly obtain accurate and reliable results. From scanning experiments to flow dynamics analysis, the Evolution 260 Bio with INSIGHT software provides maximum flexibility to meet different needs.
Collect the full spectrum after each measurement to enhance analysis
We have industry-leading data acquisition speed, capable of obtaining 100 data points per second.If you use an 8-link pool converter, you can obtain 160 data points per second
Using multi-level curve fitting and continuous reaction mode for comprehensive data fitting, it canEasily analyze complex data
By utilizing precise electronic triggering and high-speed hybrid accessories, you can achieve zeroSecond level dynamic measurement
• Use complex mathematical analysis functions such as derivatives and smoothing to convert data into what you needformat
Using the merge workbook function to integrate data into one workbook can quickly achieveConveniently compare data from multiple experiments
Comprehensive solution for DNA melting curve:
Whether you are detecting DNA/RNA sequences, double stranded or triple helix sequences, Evolution 260 Bio can meet your experimental needs.
Accurate control experiment using a multi-stage heating/cooling curve with a slope of 0.4-20 ° C/min
• Use built-in fitting algorithms to automatically calculate Tm values for different melting curves
Optional Peltier single cell and 8-cell with temperature range of 0 ° C to 110 ° C to meet your laboratory needsdemand
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